The analysis of macromolecular interactions by sedimentation equilibrium
نویسندگان
چکیده
منابع مشابه
Macromolecular characterization by sedimentation equilibrium in the preparative ultracentrifuge.
متن کامل
Analysis of sedimentation equilibrium distributions reflecting nonideal macromolecular associations.
A rigorous statistical-mechanical approach is adopted to derive general quantitative expressions that allow for the effects of thermodynamic nonideality in equilibrium measurements reflecting interaction between dissimilar macromolecular reactants. An analytical procedure based on these expressions is then formulated for obtaining global estimates of equilibrium constants and the corresponding ...
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Determination of the assembly state(s) of a membrane protein poses a difficult analytical problem. A powerful technique that is well adapted to meet this challenge is solution interaction analysis using analytical ultracentrifugation. Due to their hydrophobic nature, membrane proteins require detergent (or lipid) for solubilization in solution. Thus, a fundamental obstacle to be overcome in det...
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Determination of the assembly state(s) of a membrane protein poses a difficult analytical problem. A powerful technique that is well adapted to meet this challenge is solution interaction analysis using analytical ultracentrifugation. Due to their hydrophobic nature, membrane proteins require detergent (or lipid) for solubilization in solution. Thus, a fundamental obstacle to be overcome in det...
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since esp received universal attention to smooth the path for academic studies and productions, a great deal of research and studies have been directed towards this area. swales’ (1990) model of ra introduction move analysis has served a pioneering role of guiding many relevant studies and has proven to be productive in terms of helpful guidelines that are the outcome of voluminous productions ...
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ژورنال
عنوان ژورنال: Methods
سال: 2011
ISSN: 1046-2023
DOI: 10.1016/j.ymeth.2010.12.005